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1. Cloning and Characterization of a Genomic Clone of the Root Bud Growth-Response Gene Gro7 from Leafy Spurge (Euphorbia esula L.). D.P. Horvath, U.S. Department of Agriculture, Agricultural Research Service, Biosciences Research Laboratory, State University Station, Fargo, ND 58105-5674.
One of the key features of leafy spurge is the presence of a large number of below ground shoot meristems located along the roots of the plant (referred to as root buds) which remain dormant until they are separated from the aerial portion of the plant. Previous results indicated that loss of contact to the aerial plant parts results in changes in gene expression in the cells of the root buds. We have reported cloning of a partial cDNA for one of the genes, Gro7. Here, we report the cloning of a partial genomic clone of the Gro7 gene. A genomic library (of 2 x 106 primary pfu with an average insert size of -6 kilobases) was constructed. This library was screened by hybridization to the Gro7 cDNA clone. Eight out of 500,000 plaques hybridized to the cDNA. Since the Gro7 gene is a single copy gene, this result indicates that the leafy spurge genome is roughly 3.75 x 108 bp/haploid genome. One of the genomic clones was further purified and characterized by restriction mapping. This clone is currently being sequenced in hopes of gaining information concerning the function of this gene.
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